GenSpin™ Basket
Collect an oral sample in one minute with maximum absorption and extraction.
- Fits most microcentrifuge tubes
- Contoured design recovers over 95% of sample
- Isolates large fragment sizes of DNA
Cat. No. | Quantity |
---|---|
G-1102-100 | 100 per package |
The GenSpin Basket is used for separating small volumes of fluids from collection swabs, such as the Gentueri CollectEject™ Oral, Large Oral, Touch DNA and Blade Swabs. Typical DNA applications include genetic, forensic, human identity, animal diagnostic and environmental DNA testing. The spin baskets are ideal for pre-analytical cell lysis and DNA extraction methods for Next-Gen and Sanger Sequencing, STR and qPCR protocols. The GenSpin Basket design features a contoured bottom which directs 95% of the sample through the large openings during centrifugation. This, coupled with the ability to be spun at a lower speed than current spin baskets allows for the isolation of large fragment sizes of DNA.
- Assemble a GenSpin Basket into a standard 2.0mL centrifuge tube with hinged cap (not provided).
- Collect a CollectEject™ Swab sample according to collection instructions.
- Eject the CollectEject™ sample tip into a GenSpin Basket.
- Add lysis buffer to the GenSpin Basket, close the tube and incubate according to your established protocols to lyse the cells. We recommend 300 μL lysis buffer for optimum DNA results.
- After lysis incubation, centrifuge the GenSpin Basket/microcentrifuge tube assembly for 2 minutes at 1,000 rpm.
- Remove the GenSpin Basket with swab tip and discard
- Process the flow through lysate from the bottom of microcentrifuge tube according to your established
For Technical Support, Contact Us (608) 497-0366 or [email protected].
Instructions
- CollectEject Oral Sample Collection Instructions with GenDry Pouch
- CollectEject Oral Sample Processing Insructions
- Manual processing: Promega ReliaPrep™ gDNA Tissue Miniprep System
- Automated processing: Maxwell® RSC Buccal Swab DNA Kit
- Automated processing: PrepFiler Express™ Forensic DNA Extraction Kit by Applied Biosystems™
How to collect an oral sample
https://youtu.be/eGKrdd_NJCw
Technical Information
Pißarreck M, Moreira F, Foley M. Evaluation of Maximum DNA Yield from a New Noninvasive Buccal Collection Device Following Various Extraction Protocols.Genet Test Mol Biomarkers. 2021 May;25(5):376-380. doi: 10.1089/gtmb.2020.0341. Epub 2021 Apr 29. PMID: 33926219.
Application notes
- Evaluation of Performance of gDNA Extraction from CollectEject™ Swabs and PCR/Sanger Sequencing Analysis; a Comparison with Leading Swab Device
- Optimizing DNA Yield from Buccal Samples with CollectEject™ Oral Swabs Using Currently Available Extraction
- Automated Purification of DNA from Swabs using the Gentueri Oral Collection Kit by Promega